Global change impacts on arid zone ecosystems: Seedling establishment processes are threatened by temperature and water stress.

Recruitment for many arid-zone plant species is expected to be impacted by the projected increase in soil temperature and prolonged droughts associated with global climate change. As seed dormancy is considered a strategy to avoid unfavorable conditions, understanding the mechanisms underpinning vulnerability to these factors is critical for plant recruitment in intact communities, as well as for restoration efforts in arid ecosystems. This study determined the effects of temperature and water stress on recruitment processes in six grass species in the genus Triodia R.Br. from the Australian arid zone. Experiments in controlled environments were conducted on dormant and less-dormant seeds at constant temperatures of 25°C, 30°C, 35°C, and 40°C, under well-watered (Ψsoil = -0.15 MPa) and water-limited (Ψsoil = -0.35 MPa) conditions. Success at three key recruitment stages-seed germination, emergence, and survival-and final seed viability of ungerminated seeds was assessed. For all species, less-dormant seeds germinated to higher proportions under all conditions; however, subsequent seedling emergence and survival were higher in the more dormant seed treatment. An increase in temperature (35-40°C) under water-limited conditions caused 95%-100% recruitment failure, regardless of the dormancy state. Ungerminated seeds maintained viability in dry soil; however, when exposed to warm (30-40°C) and well-watered conditions, loss of viability was greater from the less-dormant seeds across all species. This work demonstrates that the transition from seed to established seedling is highly vulnerable to microclimatic constraints and represents a critical filter for plant recruitment in the arid zone. As we demonstrate temperature and water stress-driven mortality between seeds and established seedlings, understanding how these factors influence recruitment in other arid-zone species should be a high priority consideration for management actions to mitigate the impacts of global change on ecosystem resilience. The knowledge gained from these outcomes must be actively incorporated into restoration initiatives.

Seed encrusting with salicylic acid: A novel approach to improve establishment of grass species in ecological restoration.

To achieve global ambitions in large scale ecological restoration, there is a need for approaches that improve the efficiency of seed-based interventions, particularly in overcoming the bottleneck in the transition from germination to seedling establishment. In this study, we tested a novel seed-based application of the plant stress modulator compound salicylic acid as a means to reduce seedling losses in the seed-to-seedling phase. Seed coating technology (encrusting) was developed as a precursor for optimising field sowing for three grass species commonly used in restoration programs, Austrostipa scabra, Microlaena stipoides, and Rytidosperma geniculatum. Salicylic acid (SA, 0.1mM) was delivered to seeds via imbibition and seed encrusting. The effects of SA on seed germination were examined under controlled water-limited conditions (drought resilience) in laboratory setting and on seed germination, seedling emergence, seedling growth and plant survival in field conditions. Salicylic acid did not impact germination under water stress in controlled laboratory conditions and did not affect seedling emergence in the field. However, seedling survival and growth were improved in plants grown from SA treated seeds (imbibed and encrusted) under field conditions. When SA delivery methods of imbibing and coating were compared, there was no significant difference in survival and growth, showing that seed coating has potential to deliver SA. Effect of intraspecific competition as a result of seedling density was also considered. Seedling survival over the dry summer season was more than double at low seedling density (40 plants/m2) compared to high seedling density (380 plants/m2). Overall, adjustment of seeding rate according to expected emergence combined with the use of salicylic acid via coating could improve seed use efficiency in seed-based restoration.

Initiating pedogenesis of magnetite tailings using Lupinus angustifolius (narrow-leaf lupin) as an ecological engineer to promote native plant establishment.

Mine tailings pose physical and chemical challenges for plant establishment. Our aim was to learn from natural processes in long-term soil and ecosystem development to use tailings as novel parent materials and pioneer ecological-engineering plant species to ameliorate extreme conditions of tailings, and facilitate the establishment of subsequent native plants. A glasshouse trial was conducted using magnetite tailings containing various amendments, investigating the potential of the nitrogen (N)-fixing, non-native pioneer species Lupinus angustifolius (Fabaceae), narrow-leaf lupin, as a potential eco-engineer to promote soil formation processes, and whether amendment type or the presence of pioneer vegetation improved the subsequent establishment and growth of 40 species of native plants. We found that L. angustifolius eco-engineered the mine tailings, by enhancing the N status of tailings and mobilising primary mineral P into organic P via a carboxylate-exudation strategy, thereby enabling subsequent growth of native species. The substantial increases of the soil organic P (from ca. 10 to 150 mg kg-1) pool and organo-bound Al minerals (from 0 to 2 mg kg-1) were particularly evident, indicating the initiation of pedogenesis in mine tailings. Our findings suggest that the annual legume L. angustifolius has eco-engineering potential on mine tailings through N-fixation and P-mobilisation, promoting the subsequent growth of native plants. We proposed Daviesia (Fabaceae) species as native species alternatives for the non-native L. angustifolius in the Western Australian context. Our findings are important for restoration practitioners tasked with mine site restoration in terms of screening pioneer eco-engineering plant species, where native plants are required to restore after mine operations.

An ecological perspective on 'plant carnivory beyond bogs': nutritional benefits of prey capture for the Mediterranean carnivorous plant Drosophyllum lusitanicum.

BACKGROUND AND AIMS: Little is known about the evolutionary and ecological drivers of carnivory in plants, particularly for those terrestrial species that do not occur in typical swamp or bog habitats. The Mediterranean endemic Drosophyllum lusitanicum (Drosophyllaceae) is one of very few terrestrial carnivorous plant species outside of Australia to occur in seasonally dry, fire-prone habitats, and is thus an ecological rarity. Here we assess the nutritional benefits of prey capture for D. lusitanicum under differing levels of soil fertility in situ. METHODS: We measured the total nitrogen and stable nitrogen and carbon isotope ratios of D. lusitanicum leaves, neighbouring non-carnivorous plant leaves, and groups of insect prey in three populations in southern Spain. We calculated trophic enrichment (ε15N) and estimated the proportion of prey-derived nitrogen (%Nprey) in D. lusitanicum leaves, and related these factors to soil chemistry parameters measured at each site. KEY RESULTS: In all three populations studied, D. lusitanicum plants were significantly isotopically enriched compared with neighbouring non-carnivorous plants. We estimated that D. lusitanicum gain ~36 %Nprey at the Puerto de Gáliz site, ~54 %Nprey at the Sierra Carbonera site and ~75 %Nprey at the Montera del Torero site. Enrichment in N isotope (ε15N) differed considerably among sites; however, it was not found to be significantly related to log10(soil N), log10(soil P) or log10(soil K). CONCLUSIONS: Drosophyllum lusitanicum individuals gain a significant nutritional benefit from captured prey in their natural habitat, exhibiting proportions of prey-derived nitrogen that are similar to those recorded for carnivorous plants occurring in more mesic environments. This study adds to the growing body of literature confirming that carnivory is a highly beneficial nutritional strategy not only in mesic habitats but also in seasonally dry environments, and provides insights to inform conservation strategies for D. lusitanicum in situ.

Geochemical and mineralogical constraints in iron ore tailings limit soil formation for direct phytostabilization.

The present study aimed to characterize key physico-chemical and mineralogical attributes of magnetite iron (Fe) ore tailings to identify potential constraints limiting in situ soil formation and direct phytostabilization. Tailings of different age, together with undisturbed local native soils, were sampled from a magnetite mine in Western Australia. Tailings were extremely alkaline (pH > 9.0), with a lack of water stable aggregate and organic matter, and contained abundant primary minerals including mica (e.g., biotite), with low specific surface area (N2-BET around 1.2 m2 g-1). These conditions remained relatively unchanged after four years' aging under field conditions. Chemical extraction and spectroscopic analysis [e.g., X-ray diffraction (XRD) and synchrotron-based Fe K edge X-ray absorption fine structure spectroscopy (XAFS) analysis] revealed that the aging process decreased biotite-like minerals, but increased hematite and magnetite in the tailings. However, the aged tailings lacked goethite, a compound abundant in natural soils. Examination using backscattered-scanning electron microscope - energy dispersive X-ray spectrometry (BSE-SEM-EDS) revealed that aged tailings contained discrete sharp edged Fe-bearing minerals that did not physically integrate with other minerals (e.g., Si/Al bearing minerals). In contrast, Fe minerals in native soils appeared randomly distributed and closely amassed with Si/Al rich phyllosilicates, with highly eroded edges. The lack of labile organic matter and the persistence of alkaline-saline conditions may have significantly hindered the bioweathering of Fe-minerals and the biogenic formation of secondary Fe-minerals in tailings. However, there is signature that a native pioneer plant, Maireana brevifolia can facilitate the bioweathering of Fe-bearing minerals in tailings. We propose that eco-engineering inputs like organic carbon accumulation, together with the introduction of functional microbes and pioneer plants, should be adopted to accelerate bioweathering of Fe-bearing minerals as a priority for initiating in situ soil formation in the Fe ore tailings.

Ecological niche and bet-hedging strategies for Triodia (R.Br.) seed germination.

Background and Aims: Regeneration dynamics in many arid zone grass species are regulated by innate seed dormancy mechanisms and environmental cues (temperature, moisture and fire) that result in infrequent germination following rainfall. This study investigated bet-hedging strategies associated with dormancy and germination in arid zone Triodia species from north-west Australia, by assessing (1) the effects of the mechanical restriction imposed by the indehiscent floral bracts (i.e. floret) covering the seed and (2) the impact of dormancy alleviation on florets and cleaned seeds (i.e. florets removed) when germinated under water stress. Methods: The initial dormancy status and germination for six species were tested on intact florets and cleaned seeds, across temperatures (10-40 °C) with and without the fire-related stimulant karrikinolide (KAR1), and under alternating light or constant dark conditions. Physiological dormancy alleviation was assessed by wet/dry cycling florets over a period of 10 weeks, and germination was compared against untreated florets, and cleaned seeds across a water potential gradient between 0 and -1.5 MPa. Key Results: Florets restricted germination (<45 %) at all temperatures and, despite partial alleviation of physiological dormancy (wet/dry cycling for 8 weeks), intact florets germinated only at high water potentials. Cleaned seeds showed the highest germination (40-90 %) across temperatures when treated with KAR1, and germinated at much lower water potentials (-0.4 and -0.9 MPa). Triodia pungens was the most responsive to KAR1, with both seeds and florets responding, while for the remaining five species, KAR1 had a positive effect for seeds only. Conclusions: Only after seed dormancy was alleviated by removing florets and when KAR1 was applied did germination under water stress increase. This suggests that seeds of these Triodia species are cued to recruit following fire and during periods of high precipitation. Climate change, driven by large shifts in rainfall patterns, is likely to impact Triodia recruitment further in arid zone grasslands.

Seedling mortality during biphasic drought in sandy Mediterranean soils.

Climate change is increasing the frequency and intensity of drought, and seedling response to a recurrent pattern of drought stress is necessary to understand vegetation establishment patterns in particularly for ecological restoration and conservation projects. A controlled environment study investigated seedling physiological response of framework Mediterranean tree species to simulated successive droughts. Six-month-old seedlings were grown in 1.0m tall pots to emulate deep soil profiles and subjected to a well watered treatment and a drought treatment consisting of an initial 60 day drought (water withholding), followed by 120 days of re-watering and a subsequent 60 day drought. Soil water access, soil water content, maximum root depth and xylem water potential were assessed through successive harvests. To assess seedling response to multiple droughts, gas-exchange and chlorophyll fluorescence measurements were taken every 15 days after each drought, and multiple times throughout re-watering. No seedling mortality was observed during the initial drought, whereas 100% mortality of all species occurred within 48 days of the second drought. Seedling gas exchange and water potential decreased with decreasing water availability but was dependent on the isohydric or anisohydric behaviour of individual species. An absence of sustained photoprotection during the second drought phase heightened photodamage to foliar tissues resulting in a more rapid decrease of gs and leaf water potential. Therefore, biphasic drought proved detrimental to seedling establishment by reducing physiological resilience, highlighting the severity of future climate change predictions towards the regeneration capacity of Mediterranean ecosystems.

Seeds of Brassicaceae weeds have an inherent or inducible response to the germination stimulant karrikinolide.

BACKGROUND AND AIMS: Karrikinolide (KAR(1)) is a smoke-derived chemical that can trigger seeds to germinate. A potential application for KAR(1) is for synchronizing the germination of weed seeds, thereby enhancing the efficiency of weed control efforts. Yet not all species germinate readily with KAR(1), and it is not known whether seemingly non-responsive species can be induced to respond. Here a major agronomic weed family, the Brassicaceae, is used to test the hypothesis that a stimulatory response to KAR(1) may be present in physiologically dormant seeds but may not be expressed under all circumstances. METHODS: Seeds of eight Brassicaceae weed species (Brassica tournefortii, Raphanus raphanistrum, Sisymbrium orientale, S. erysimoides, Rapistrum rugosum, Lepidium africanum, Heliophila pusilla and Carrichtera annua) were tested for their response to 1 µm KAR(1) when freshly collected and following simulated and natural dormancy alleviation, which included wet-dry cycling, dry after-ripening, cold and warm stratification and a 2 year seed burial trial. KEY RESULTS: Seven of the eight Brassicaceae species tested were stimulated to germinate with KAR(1) when the seeds were fresh, and the remaining species became responsive to KAR(1) following wet-dry cycling and dry after-ripening. Light influenced the germination response of seeds to KAR(1), with the majority of species germinating better in darkness. Germination with and without KAR(1) fluctuated seasonally throughout the seed burial trial. CONCLUSIONS: KAR(1) responses are more complex than simply stating whether a species is responsive or non-responsive; light and temperature conditions, dormancy state and seed lot all influence the sensitivity of seeds to KAR(1), and a response to KAR(1) can be induced. Three response types for generalizing KAR(1) responses are proposed, namely inherent, inducible and undetected. Given that responses to KAR(1) were either inherent or inducible in all 15 seed lots included in this study, the Brassicaceae may be an ideal target for future application of KAR(1) in weed management.

Prior hydration of Brassica tournefortii seeds reduces the stimulatory effect of karrikinolide on germination and increases seed sensitivity to abscisic acid.

BACKGROUND AND AIMS: The smoke-derived compound karrikinolide (KAR(1)) shows significant potential as a trigger for the synchronous germination of seeds in a variety of plant-management contexts, from weed seeds in paddocks, to native seeds when restoring degraded lands. Understanding how KAR(1) interacts with seed physiology is a necessary precursor to the development of the compound as an efficient and effective management tool. This study tested the ability of KAR(1) to stimulate germination of seeds of the global agronomic weed Brassica tournefortii, at different hydration states, to gain insight into how the timing of KAR(1) applications in the field should be managed relative to rain events. METHODS: Seeds of B. tournefortii were brought to five different hydration states [equilibrated at 15 % relative humidity (RH), 47 % RH, 96 % RH, fully imbibed, or re-dried to 15 % RH following maximum imbibition] then exposed to 1 nm or 1 microm KAR(1) for one of five durations (3 min, 1 h, 24 h, 14 d or no exposure). KEY RESULTS: Dry seeds with no history of imbibition were the most sensitive to KAR(1); sensitivity was lower in seeds that were fully imbibed or fully imbibed then re-dried. In addition, reduced sensitivity to KAR(1) was associated with an increased sensitivity to exogenously applied abscisic acid (ABA). CONCLUSIONS: Seed water content and history of imbibition were found to significantly influence whether seeds germinate in response to KAR(1). To optimize the germination response of seeds, KAR(1) should be applied to dry seeds, when sensitivity to ABA is minimized.